| 1104837020 | A & B Ambulance Service, Inc. | Organization | Land Ambulance | Hopewell, VA |
| 1205824307 | Abbs Valley-Boissevain Pocahontas Rescue Squad Inc | Organization | Ambulance | Boissevain, VA |
| 1205819646 | Abingdon Convalescent Ambulance Service Incorporated | Organization | Ambulance | Abingdon, VA |
| 1831144831 | Accomack County Board of Supervisors | Organization | Ambulance | Parksley, VA |
| 1891868139 | Alberta Volunteer Fire Department | Organization | Land Ambulance | Alberta, VA |
| 1528013844 | Allen'S Ambulance | Organization | Land Ambulance | Dillwyn, VA |
| 1164561601 | Allen'S Ambulance | Organization | Ambulance | Dillwyn, VA |
| 1598719734 | Altavista Life Saving and First Aid Crew, Incorporated | Organization | Ambulance | Altavista, VA |
| 1780797159 | Amelia Ambulance Service Inc | Organization | Land Ambulance | Amelia Court House, VA |
| 1245287929 | Amelia Emergency Squad Inc | Organization | Land Ambulance | Amelia, VA |
| 1992752505 | American Lifeline Medical Transport Inc | Organization | Land Ambulance | South Hill, VA |
| 1033214887 | American Medical Response Mid-Atlantic Inc | Organization | Ambulance | Annandale, VA |
| 1689624637 | Amherst County Board of Supervisors | Organization | Land Ambulance | Amherst, VA |
| 1700925641 | Angelcare Ambulance Service | Organization | Ambulance | Abingdon, VA |
| 1437215704 | Appomattox County Rescue Squad Inc | Organization | Land Ambulance | Appomattox, VA |
| 1255342648 | Arlington County Government Department of Managment & Finance | Organization | Ambulance | Arlington, VA |
| 1083742035 | Augusta Health Care Inc | Organization | Ambulance | Fishersville, VA |
| 1063627446 | Bath County Community Hospital | Organization | Land Ambulance | Hot Springs, VA |
| 1659338176 | Baywood Rescue Squad | Organization | Ambulance | Galax, VA |
| 1497839716 | Beacon of Life Ambulance Service | Organization | Land Ambulance | St. Paul, VA |
| 1164647608 | Bedford County Board of Supervisors | Organization | Land Ambulance | Bedford, VA |
| 1598800666 | Big Stone Gap Rescue Squad, Inc. | Organization | Land Ambulance | Big Stone Gap, VA |
| 1144323478 | Bland County Volunteer Rescue Squad, Inc. | Organization | Land Ambulance | Bland, VA |
| 1609821792 | Bloxom Volunteer Fire Company | Organization | Land Ambulance | Bloxom, VA |
| 1699812495 | Blue Ridge Volunteer Rescue Squad Inc | Organization | Ambulance | Ararat, VA |
| 1013028158 | Bluefield Virginia Rescue Squad, Inc. | Organization | Land Ambulance | Bluefield, VA |
| 1710090014 | Botetourt County Board of Supervisors | Organization | Ambulance | Fincastle, VA |
| 1861441073 | Boydton Life Station Inc | Organization | Land Ambulance | Boydton, VA |
| 1710952460 | Bristol Life Saving Crew Inc. | Organization | Land Ambulance | Bristol, VA |
| 1952390726 | Brunswick Volunteer Rescue Squad Inc | Organization | Ambulance | Lawrenceville, VA |
| 1205949773 | Campbell County Board of Supervisors | Organization | Land Ambulance | Rustburg, VA |
| 1285613620 | Cape Charles Rescue Service Inc | Organization | Land Ambulance | Cape Charles, VA |
| 1710934617 | Carilion Clinic Patient Transportation, LLC | Organization | Air Ambulance | Roanoke, VA |
| 1922141977 | Carilion Patient Transportation Services LLC | Organization | Air Ambulance | Roanoke, VA |
| 1164648317 | Caroline County Board of Supervisors | Organization | Ambulance | Bowling Green, VA |
| 1659327625 | Carroll County Emergency Medical Services Inc | Organization | Ambulance | Hillsville, VA |
| 1124066840 | Castlewood Fire and Rescue, Inc. | Organization | Land Ambulance | Castlewood, VA |
| 1922057835 | Ccdf Volunteer Fire Department, Inc. | Organization | Land Ambulance | Stuart, VA |
| 1366635328 | Centra Health Inc | Organization | Air Ambulance | Lynchburg, VA |
| 1306031596 | Charles City County Board of Supervisors | Organization | Land Ambulance | Charles City, VA |
| 1720171945 | Childrens Hospital of the Kings Daughters Inc | Organization | Ambulance | Norfolk, VA |
| 1619950052 | Chilhowie Ambulance Service Inc | Organization | Ambulance | Abingdon, VA |
| 1104823624 | Chincoteague Volunteer Fire Co | Organization | Ambulance | Chincoteague Island, VA |
| 1922091578 | Chores and Errands Ambulance Inc | Organization | Ambulance | Raven, VA |
| 1902970593 | City of Alexandria | Organization | Ambulance | Alexandria, VA |
| 1659385938 | City of Chesapeake | Organization | Ambulance | Chesapeake, VA |
| 1336184597 | City of Colonial Heights | Organization | Ambulance | Colonial Heights, VA |
| 1194911099 | City of Fairfax | Organization | Ambulance | Fairfax, VA |
| 1770538365 | City of Franklin | Organization | Land Ambulance | Franklin, VA |
| 1972676799 | City of Fredericksburg | Organization | Ambulance | Fredericksburg, VA |